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Chemically activatable viral capsid functionalized for cancer targeting.

Authors :
Chen, Chun-Chieh
Chen, Chun-Chieh
Xing, Li
Stark, Marie
Ou, Tingwei
Holla, Prasida
Xiao, Kai
Kamita, Shizuo G
Hammock, Bruce D
Lam, Kit
Cheng, R Holland
Chen, Chun-Chieh
Chen, Chun-Chieh
Xing, Li
Stark, Marie
Ou, Tingwei
Holla, Prasida
Xiao, Kai
Kamita, Shizuo G
Hammock, Bruce D
Lam, Kit
Cheng, R Holland
Source :
Nanomedicine (London, England); vol 11, iss 4, 377-390; 1743-5889
Publication Year :
2016

Abstract

AimTo design a theranostic capsule using the virus-like nanoparticle of the hepatitis E virus modified to display breast cancer cell targeting functional group (LXY30).MethodsFive surface-exposed residues were mutated to cysteine to allow conjugation to maleimide-linked chemical groups via thiol-selective linkages. Engineered virus-like nanoparticles were then covalently conjugated to a breast cancer recognized ligand, LXY30 and an amine-coupled near-infrared fluorescence dye.ResultsLXY30-HEV VLP was checked for its binding and entry to a breast cancer cell line and for tumor targeting in vivo to breast cancer tissue in mice. The engineered virus-like nanoparticle not only targeted cancer cells, but also appeared immune silent to native hepatitis E virus antibodies due to epitope disruption at the antibody-binding site.ConclusionThese results demonstrate the production of a theranostic capsule suitable for cancer diagnostics and therapeutics based on surface modification of a highly stable virus-like nanoparticle.

Details

Database :
OAIster
Journal :
Nanomedicine (London, England); vol 11, iss 4, 377-390; 1743-5889
Notes :
application/pdf, Nanomedicine (London, England) vol 11, iss 4, 377-390 1743-5889
Publication Type :
Electronic Resource
Accession number :
edsoai.on1378689659
Document Type :
Electronic Resource