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Spatially Resolved Molecular Approaches for the Characterisation of Non-Invasive Follicular Tumours with Papillary-like Features (NIFTPs)

Authors :
Piga, I
L'Imperio, V
Principi, L
Bellevicine, C
Fusco, N
Maffini, F
Venetis, K
Ivanova, M
Seminati, D
Casati, G
Pagani, L
Galimberti, S
Capitoli, G
Garancini, M
Gatti, A
Magni, F
Pagni, F
Piga, Isabella
L'Imperio, Vincenzo
Principi, Lucrezia
Bellevicine, Claudio
Fusco, Nicola
Maffini, Fausto
Venetis, Konstantinos
Ivanova, Mariia
Seminati, Davide
Casati, Gabriele
Pagani, Lisa
Galimberti, Stefania
Capitoli, Giulia
Garancini, Mattia
Gatti, Andrea-Valer
Magni, Fulvio
Pagni, Fabio
Piga, I
L'Imperio, V
Principi, L
Bellevicine, C
Fusco, N
Maffini, F
Venetis, K
Ivanova, M
Seminati, D
Casati, G
Pagani, L
Galimberti, S
Capitoli, G
Garancini, M
Gatti, A
Magni, F
Pagni, F
Piga, Isabella
L'Imperio, Vincenzo
Principi, Lucrezia
Bellevicine, Claudio
Fusco, Nicola
Maffini, Fausto
Venetis, Konstantinos
Ivanova, Mariia
Seminati, Davide
Casati, Gabriele
Pagani, Lisa
Galimberti, Stefania
Capitoli, Giulia
Garancini, Mattia
Gatti, Andrea-Valer
Magni, Fulvio
Pagni, Fabio
Publication Year :
2023

Abstract

Noninvasive follicular thyroid neoplasms with papillary-like nuclear features (NIFTP) are low-risk thyroid lesions most often characterised by RAS-type mutations. The histological diagnosis may be challenging, and even immunohistochemistry and molecular approaches have not yet provided conclusive solutions. This study characterises a set of NIFTPs by Matrix-Assisted Laser Desorption/Ionisation (MALDI)-Mass Spectrometry Imaging (MSI) to highlight the proteomic signatures capable of overcoming histological challenges. Archived formalin-fixed paraffin-embedded samples from 10 NIFTPs (n = 6 RAS-mutated and n = 4 RAS-wild type) were trypsin-digested and analysed by MALDI-MSI, comparing their profiles to normal tissue and synchronous benign nodules. This allowed the definition of a four-peptide signature able to distinguish RAS-mutant from wild-type cases, the latter showing proteomic similarities to hyperplastic nodules. Moreover, among the differentially expressed signals, Peptidylprolyl Isomerase A (PPIA, 1505.8 m/z), which has already demonstrated a role in the development of cancer, was found overexpressed in NIFTP RAS-mutated nodules compared to wild-type lesions. These results underlined that high-throughput proteomic approaches may add a further level of biological comprehension for NIFTPs. In the future, thanks to the powerful single-cell detail achieved by new instruments, the complementary NGS-MALDI imaging sequence might be the correct methodological approach to confirm that the current NIFTP definition encompasses heterogeneous lesions that must be further characterised.

Details

Database :
OAIster
Notes :
ELETTRONICO, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1376720927
Document Type :
Electronic Resource