Comparative Analysis of Microbial Prevalence in Normal and Endometritic cows

The objective of this study was to perform a comparative analysis of microbial prevalence in normal and endometritic cows. To determine the causative microbes of endometritis, cows were divided into four groups, comprising 10 cows each. Group A: normal cows with no signs and symptoms. Group B: Young stock (fit for breeding) cows suffering from endometritis. Group C: milking cows suffering from Endometritis. Group D: dry cows suffering from Endometritis. Three sterile swabs were collected from all cows of four groups, thus making a total of 120 samples. Bacterial isolation and identification was performed according to bergey’s manual. Most common pathogen in the uterus of endometritic cows were Escherichia coli and then Trueperella pyogenes > Salmonella enterica > Klebsiella pneumonia > Bacillus subtilis > Fusobacterium necrophorum > Proteus vulgaris > Staphylococcus aureus > Streptococcus pneumoniae. In normal cows, mostly numbers of isolates were gram positive. S. aureus was found to be most abundant in normal cows followed by S. pneumoniae, B. subtilis, T. pyogenes, E. coli and K. pneumoniae. Higher numbers of bacteria were found in endometritic cows as compared to normal cows. In conclusion, E. coli was found to be abundant pathogens in the diseased cows and S. aureus was mostly found in normal cows. This study provides information about prevalence of endometritis in cows which can be used as base to treat endometritic cows.