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Modular UBE2H-CTLH E2-E3 complexes regulate erythroid maturation

Authors :
Sherpa, Dawafuti
Mueller, Judith
Karayel, Oezge
Xu, Peng
Yao, Yu
Chrustowicz, Jakub
Gottemukkala, Karthik, V
Baumann, Christine
Gross, Annette
Czarnecki, Oliver
Zhang, Wei
Gu, Jun
Nilvebrant, Johan
Sidhu, Sachdev S.
Murray, Peter J.
Mann, Matthias
Weiss, Mitchell J.
Schulman, Brenda A.
Alpi, Arno F.
Sherpa, Dawafuti
Mueller, Judith
Karayel, Oezge
Xu, Peng
Yao, Yu
Chrustowicz, Jakub
Gottemukkala, Karthik, V
Baumann, Christine
Gross, Annette
Czarnecki, Oliver
Zhang, Wei
Gu, Jun
Nilvebrant, Johan
Sidhu, Sachdev S.
Murray, Peter J.
Mann, Matthias
Weiss, Mitchell J.
Schulman, Brenda A.
Alpi, Arno F.
Publication Year :
2022

Abstract

The development of haematopoietic stem cells into mature erythrocytes - erythropoiesis - is a controlled process characterized by cellular reorganization and drastic reshaping of the proteome landscape. Failure of ordered erythropoiesis is associated with anaemias and haematological malignancies. Although the ubiquitin system is a known crucial post-translational regulator in erythropoiesis, how the erythrocyte is reshaped by the ubiquitin system is poorly understood. By measuring the proteomic landscape of in vitro human erythropoiesis models, we found dynamic differential expression of subunits of the CTLH E3 ubiquitin ligase complex that formed maturation stage-dependent assemblies of topologically homologous RANBP9- and RANBP10-CTLH complexes. Moreover, protein abundance of CTLH's cognate E2 ubiquitin conjugating enzyme UBE2H increased during terminal differentiation, and UBE2H expression depended on catalytically active CTLH E3 complexes. CRISPR-Cas9-mediated inactivation of CTLH E3 assemblies or UBE2H in erythroid progenitors revealed defects, including spontaneous and accelerated erythroid maturation as well as inefficient enucleation. Thus, we propose that dynamic maturation stage-specific changes of UBE2H-CTLH E2-E3 modules control the orderly progression of human erythropoiesis.<br />QC 20221223

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1372250684
Document Type :
Electronic Resource
Full Text :
https://doi.org/10.7554.eLife.77937