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A full-length infectious cDNA clone of a dsRNA totivirus-like virus
- Publication Year :
- 2022
-
Abstract
- Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family. Unlike yeast and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first infectious full-length cDNA clone of a totivirus-like virus, the Omono River virus (OmRV), and produced infectious particles using an RNA -transcript-based method. Compared with the parent wild-type particles from nature, the infectious-cloning OmRV particles have presented strong cytopathic effects, infectivity and similar morphology. Thus far, the established system is one of the few reported systems for generating a non-segmented dsRNA virus cDNA clone.
Details
- Database :
- OAIster
- Notes :
- Wang, Han, Maia, Filipe R.N.C., Okamoto, Kenta
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1372226518
- Document Type :
- Electronic Resource
- Full Text :
- https://doi.org/10.1016.j.virol.2022.09.010