Wistar Rats Hippocampal Neurons Response to Blood Low-Density Polyethylene Microplastics: A Pathway Analysis of SOD, CAT, MDA, 8-OHdG Expression in Hippocampal Neurons and Blood Serum Aβ42 Levels

Yudhiakuari Sincihu,1,2,* Muhammad Farid Dimjadi Lusno,2 Tri Marthy Mulyasari,2 Saliza Mohd Elias,3,* I Ketut Sudiana,4,* Kurnia Kusumastuti,4,* Lilis Sulistyorini,5 Soedjajadi Keman5,* 1Faculty of Medicine, Widya Mandala Surabaya Catholic University, Surabaya, Indonesia; 2Doctoral Program of Public Health, Universitas Airlangga, Surabaya, Indonesia; 3Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Malaysia; 4Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; 5Faculty of Public Health, Universitas Airlangga, Surabaya, Indonesia*These authors contributed equally to this workCorrespondence: Soedjajadi Keman, Faculty of Public Health, Universitas Airlangga, Ir. Soekarno Street, Mulyorejo, Surabaya City, East Java, 60115, Indonesia, Tel +62 812 1729 908, Fax +62 31 5920948, Email soedja_keman@fkm.unair.ac.idPurpose: Low-density polyethylene microplastics are ingested into the bloodstream and distributed to all the organ tissue, including the hippocampus, causing toxic effects. This research aimed to elucidate the responses of hippocampal neurons to microplastic in the blood based on the expressions of superoxide dismutase (SOD), catalase (CAT) enzymes, malondialdehyde (MDA), 8-oxo-7,8-dihydro-2-deoxyguanosine (8-OHdG) in hippocampal neurons, and blood serum amyloid beta 1– 42 (Aβ 42) levels using SMART PLS pathway analysis.Methods: This was a pure experimental research on Wistar rats with a post-test control group design. Five experimental groups (X1, X2, X3, X4, X5) were given 0.0375 mg, 0.075 mg, 0.15 mg, 0.3 mg, and 0.6 mg of low-density polyethylene microplastics mixed in 2cc distilled water, respectively. Furthermore, except for control (C), the groups received microplastics an oral probe for 90 days.Results: The molecular response of hippocampal neurons of Wistar rats to microplastics in the blood significantly decreased SOD enzyme expression, while CAT enzyme was unaffected. It considerably increased n