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Selective staining of rodent acrosomes with phosphotungstic acid
- Publication Year :
- 1975
-
Abstract
- The acrosome of rodents, as well as the acrosomal granule,the head cap and the elastic materials fixed in glutaraldehyde and embedded in EPON, show a high degree of affinity for aqueous phosphotungstic acid (PTA). Under the electron microscope the reactive material shows up in strong contrast when fine sections have been floated on aequous 3-5 % PTA. In thick PTA-treated sections, the PTA can be easily located by light microscopy by subsequent staining with methyl green pyronin or UNNA's blue.
Details
- Database :
- OAIster
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1348916524
- Document Type :
- Electronic Resource