Inflammation and Post-traumatic Headache after Closed Head Impact Model of Engineered Rotational Acceleration (CHIMERA) Injury in Rats

Inflammation and Post-traumatic Headache after Closed Head Impact Model of Engineered Rotational Acceleration (CHIMERA) Injury in Rats Haley Spencera, Kennett Radfordb, Martin Boeseb, Rina Bermanc, Sharon Kima, Yeonho Kimd,e, Amanda Fud,e, Joseph McCabea,d,e, and Kwang Choia,b,c,f a Program in Neuroscience, Uniformed Services University, Bethesda, MD b Daniel K. Inouye Graduate School of Nursing, Uniformed Services University, Bethesda, MD cCenter for the Study of Traumatic Stress, Uniformed Services University, Bethesda, MD dPreclinical Behavior and Models Core, Department of Laboratory Animal Resources, Uniformed Services University, Bethesda, MD eDepartment of Anatomy, Physiology & Genetics, F. E. Hébert School of Medicine, Uniformed Services University, Bethesda, MD fDepartment of Psychiatry, F. E. Hébert School of Medicine, Uniformed Services University, Bethesda, MD The opinions, interpretations, conclusions and recommendations are those of the authors and are not necessarily endorsed by the U.S. Army, Department of Defense, the U.S. Government or the Uniformed Services University of the Health Sciences. The use of trade names does not constitute an official endorsement or approval of the use of reagents or commercial hardware or software. This document may not be cited for purposes of advertisement. The authors have declared that no competing interests exist. The CHIMERA device mimics the biomechanics of an impact injury and the resulting free head motion. Haar, C.V. et al. Experimental neurology, 2019 IV jugular venous catheters: our approach Schaeffer, C. R., Woods, K. M., & Longo, G. M. (2014). Rat jugular catheter model of biofilm-mediated infection. In Staphylococcus Epidermidis (pp. 199-206). Humana Press, Totowa, NJ. https://www.instechlabs.com/applications/blood-sampling/rat-microsampling 4 Periorbital and hind paw von Frey testing for mechanical allodynia Experimental Design -1d Baseline 1d Blood Activity von Frey CHIMERA 0d Blood 2 4 6 Hours 3d 7d 8
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