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Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain
- Source :
- Brown , J M , Phan , B A , Aalling , N , Morton , G J , Schwartz , M W & Scarlett , J M 2022 , ' Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain ' , STAR Protocols , vol. 3 , no. 2 , 101329 .
- Publication Year :
- 2022
-
Abstract
- Intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) elicits remission of diabetic hyperglycemia in rodent models of type 2 diabetes. Here, we present an optimized protocol to study the intracellular signaling pathways underlying the FGF1-induced sustained glucose lowering in the mouse brain. This protocol combines icv injection of FGF1 and osmotic mini-pump infusion of U0126, an inhibitor of MAPK/ERK signaling. We describe the surgical procedure and verification of U0126 inhibition of FGF1-stimulated hypothalamic MAPK/ERK signaling via western blot. For complete details on the use and execution of this protocol, please refer to Brown et al. (2021).
Details
- Database :
- OAIster
- Journal :
- Brown , J M , Phan , B A , Aalling , N , Morton , G J , Schwartz , M W & Scarlett , J M 2022 , ' Combined micro-osmotic pump infusion and intracerebroventricular injection to study FGF1 signaling pathways in the mouse brain ' , STAR Protocols , vol. 3 , no. 2 , 101329 .
- Notes :
- application/pdf, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1340143461
- Document Type :
- Electronic Resource