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Industry Viable Electrochemical DNA Detection Sensor Architecture via a Stem-Loop Methylene Blue Redox Reporter and Rapid In Situ Probe Immobilization Method for Pharmacogenetic Biomarker Testing Application

Authors :
Jayawardena, A
Tan, SM
Richardson, MB
Chan, J
Thissen, H
Voelcker, NH
Kwan, P
Jayawardena, A
Tan, SM
Richardson, MB
Chan, J
Thissen, H
Voelcker, NH
Kwan, P
Source :
18th International Meeting on Chemical Sensors (IMCS)
Publication Year :
2022

Abstract

Identification of biomarkers in clinical applications for diagnostics at the point-of-care (POC) setting requires the development of industry viable biosensing platform. Herein, we report such development of biosensor architecture for the detection of pharmacogenetic biomarker HLA-B*15:02 gene. The biosensor architecture comprises of an oligonucleotide stem-loop probe modified with a methylene blue redox (MB) reporter, immobilized via a rapid “printing” method on the commercially available disposable screen-printed electrodes (SPE). The square wave voltammetric measurements on the DNA sensor showed a clear peak difference of ∼80 nA with a significant difference in peak height values of the faradaic current generated for the MB redox moiety between the positive control (biotin-modified 19 based oligonucleotides with the sequence mimicking the specific region of the HLA-B*15:02 allele and complementary to the probe sequence) and negative control samples (biotin-modified 19 based oligonucleotides with the sequence unrelated to the probe sequence and the HLA-B*15:02 allele). These initial proof of concept results provide support for the possibility of using this signal-off biosensor architecture in the intended pharmacogenetic biomarker testing.

Details

Database :
OAIster
Journal :
18th International Meeting on Chemical Sensors (IMCS)
Publication Type :
Electronic Resource
Accession number :
edsoai.on1340014986
Document Type :
Electronic Resource