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Sarcoma classification by DNA methylation profiling

Authors :
Koelsche, C
Schrimpf, D
Stichel, D
Sill, M
Sahm, F
Reuss, DE
Blattner, M
Worst, B
Heilig, CE
Beck, K
Horak, P
Kreutzfeldt, S
Paff, E
Stark, S
Johann, P
Selt, F
Ecker, J
Sturm, D
Pajtler, K W
Reinhardt, A
Wefers, AK
Sievers, P
Ebrahimi, A
Suwala, A
Fernandez-Klett, F
Casalini, B
Korshunov, A
Hovestadt, V
Kommoss, FKF
Kriegsmann, M
Schick, M
Bewerunge-Hudler, M
Milde, T
Witt, O
Kulozik, AE
Kool, M
Romero-Perez, L
Grunewald, TGP
Kirchner, T
Wick, W
Platten, M
Unterberg, A
Uhl, M
Abdollahi, A
Debus, J
Lehner, B
Thomas, C
Hasselblatt, M
Paulus, W
Hartmann, C
Staszewski, O
Prinz, M
Hench, J
Frank, S
Versleijen-Jonkers, YMH
Weidema, ME
Mentzel, T
Griewank, K
Alava, E
Martin, JD
Gastearena, MAI
Chang, KTE
Low, SYY
Cuevas-Bourdier, A
Mittelbronn, M
Mynarek, M
Rutkowski, S
Schuller, U
Mautner, VF
Schittenhelm, J
Serrano, J
Snuderl, M
Buttner, R
Klingebiel, T
Buslei, R
Gessler, M
Wesseling, P
Dinjens, Winand
Brandner, S
Jaunmuktane, Z
Lyskjaer, I
Schirmacher, P
Stenzinger, A
Brors, B
Glimm, H
Heining, C
Tirado, OM
Sainz-Jaspeado, M
Mora, J
Alonso, J
del Muro, XG
Moran, S
Esteller, M
Benhamida, JK
Ladanyi, M
Wardelmann, E
Antonescu, C
Flanagan, A
Dirksen, U
Hohenberger, P
Baumhoer, D
Hartmann, W
Vokuhl, C
Flucke, U
Petersen, I
Mechtersheimer, G
Capper, D
Jones, DT
Frohling, S
Pfister, SM
von Deimling, A
Koelsche, C
Schrimpf, D
Stichel, D
Sill, M
Sahm, F
Reuss, DE
Blattner, M
Worst, B
Heilig, CE
Beck, K
Horak, P
Kreutzfeldt, S
Paff, E
Stark, S
Johann, P
Selt, F
Ecker, J
Sturm, D
Pajtler, K W
Reinhardt, A
Wefers, AK
Sievers, P
Ebrahimi, A
Suwala, A
Fernandez-Klett, F
Casalini, B
Korshunov, A
Hovestadt, V
Kommoss, FKF
Kriegsmann, M
Schick, M
Bewerunge-Hudler, M
Milde, T
Witt, O
Kulozik, AE
Kool, M
Romero-Perez, L
Grunewald, TGP
Kirchner, T
Wick, W
Platten, M
Unterberg, A
Uhl, M
Abdollahi, A
Debus, J
Lehner, B
Thomas, C
Hasselblatt, M
Paulus, W
Hartmann, C
Staszewski, O
Prinz, M
Hench, J
Frank, S
Versleijen-Jonkers, YMH
Weidema, ME
Mentzel, T
Griewank, K
Alava, E
Martin, JD
Gastearena, MAI
Chang, KTE
Low, SYY
Cuevas-Bourdier, A
Mittelbronn, M
Mynarek, M
Rutkowski, S
Schuller, U
Mautner, VF
Schittenhelm, J
Serrano, J
Snuderl, M
Buttner, R
Klingebiel, T
Buslei, R
Gessler, M
Wesseling, P
Dinjens, Winand
Brandner, S
Jaunmuktane, Z
Lyskjaer, I
Schirmacher, P
Stenzinger, A
Brors, B
Glimm, H
Heining, C
Tirado, OM
Sainz-Jaspeado, M
Mora, J
Alonso, J
del Muro, XG
Moran, S
Esteller, M
Benhamida, JK
Ladanyi, M
Wardelmann, E
Antonescu, C
Flanagan, A
Dirksen, U
Hohenberger, P
Baumhoer, D
Hartmann, W
Vokuhl, C
Flucke, U
Petersen, I
Mechtersheimer, G
Capper, D
Jones, DT
Frohling, S
Pfister, SM
von Deimling, A
Source :
Koelsche , C , Schrimpf , D , Stichel , D , Sill , M , Sahm , F , Reuss , DE , Blattner , M , Worst , B , Heilig , CE , Beck , K , Horak , P , Kreutzfeldt , S , Paff , E , Stark , S , Johann , P , Selt , F , Ecker , J , Sturm , D , Pajtler , K W , Reinhardt , A , Wefers , AK , Sievers , P , Ebrahimi , A , Suwala , A , Fernandez-Klett , F , Casalini , B , Korshunov , A , Hovestadt , V , Kommoss , FKF , Kriegsmann , M , Schick , M , Bewerunge-Hudler , M , Milde , T , Witt , O , Kulozik , AE , Kool , M , Romero-Perez , L , Grunewald , TGP , Kirchner , T , Wick , W , Platten , M , Unterberg , A , Uhl , M , Abdollahi , A , Debus , J , Lehner , B , Thomas , C , Hasselblatt , M , Paulus , W , Hartmann , C , Staszewski , O , Prinz , M , Hench , J , Frank , S , Versleijen-Jonkers , YMH , Weidema , ME , Mentzel , T , Griewank , K , Alava , E , Martin , JD , Gastearena , MAI , Chang , KTE , Low , SYY , Cuevas-Bourdier , A , Mittelbronn , M , Mynarek , M , Rutkowski , S , Schuller , U , Mautner , VF , Schittenhelm , J , Serrano , J , Snuderl , M , Buttner , R , Klingebiel , T , Buslei , R , Gessler , M , Wesseling , P , Dinjens , W , Brandner , S , Jaunmuktane , Z , Lyskjaer , I , Schirmacher , P , Stenzinger , A , Brors , B , Glimm , H , Heining , C , Tirado , OM , Sainz-Jaspeado , M , Mora , J , Alonso , J , del Muro , XG , Moran , S , Esteller , M , Benhamida , JK , Ladanyi , M , Wardelmann , E , Antonescu , C , Flanagan , A , Dirksen , U , Hohenberger , P , Baumhoer , D , Hartmann , W , Vokuhl , C , Flucke , U , Petersen , I , Mechtersheimer , G , Capper , D , Jones , DT , Frohling , S , Pfister , SM & von Deimling , A 2021 , ' Sarcoma classification by DNA methylation profiling ' , Nature Communications , vol. 12 , no. 1 , 498 .
Publication Year :
2021

Abstract

Sarcomas are malignant soft tissue and bone tumours affecting adults, adolescents and children. They represent a morphologically heterogeneous class of tumours and some entities lack defining histopathological features. Therefore, the diagnosis of sarcomas is burdened with a high inter-observer variability and misclassification rate. Here, we demonstrate classification of soft tissue and bone tumours using a machine learning classifier algorithm based on array-generated DNA methylation data. This sarcoma classifier is trained using a dataset of 1077 methylation profiles from comprehensively pre-characterized cases comprising 62 tumour methylation classes constituting a broad range of soft tissue and bone sarcoma subtypes across the entire age spectrum. The performance is validated in a cohort of 428 sarcomatous tumours, of which 322 cases were classified by the sarcoma classifier. Our results demonstrate the potential of the DNA methylation-based sarcoma classification for research and future diagnostic applications.

Details

Database :
OAIster
Journal :
Koelsche , C , Schrimpf , D , Stichel , D , Sill , M , Sahm , F , Reuss , DE , Blattner , M , Worst , B , Heilig , CE , Beck , K , Horak , P , Kreutzfeldt , S , Paff , E , Stark , S , Johann , P , Selt , F , Ecker , J , Sturm , D , Pajtler , K W , Reinhardt , A , Wefers , AK , Sievers , P , Ebrahimi , A , Suwala , A , Fernandez-Klett , F , Casalini , B , Korshunov , A , Hovestadt , V , Kommoss , FKF , Kriegsmann , M , Schick , M , Bewerunge-Hudler , M , Milde , T , Witt , O , Kulozik , AE , Kool , M , Romero-Perez , L , Grunewald , TGP , Kirchner , T , Wick , W , Platten , M , Unterberg , A , Uhl , M , Abdollahi , A , Debus , J , Lehner , B , Thomas , C , Hasselblatt , M , Paulus , W , Hartmann , C , Staszewski , O , Prinz , M , Hench , J , Frank , S , Versleijen-Jonkers , YMH , Weidema , ME , Mentzel , T , Griewank , K , Alava , E , Martin , JD , Gastearena , MAI , Chang , KTE , Low , SYY , Cuevas-Bourdier , A , Mittelbronn , M , Mynarek , M , Rutkowski , S , Schuller , U , Mautner , VF , Schittenhelm , J , Serrano , J , Snuderl , M , Buttner , R , Klingebiel , T , Buslei , R , Gessler , M , Wesseling , P , Dinjens , W , Brandner , S , Jaunmuktane , Z , Lyskjaer , I , Schirmacher , P , Stenzinger , A , Brors , B , Glimm , H , Heining , C , Tirado , OM , Sainz-Jaspeado , M , Mora , J , Alonso , J , del Muro , XG , Moran , S , Esteller , M , Benhamida , JK , Ladanyi , M , Wardelmann , E , Antonescu , C , Flanagan , A , Dirksen , U , Hohenberger , P , Baumhoer , D , Hartmann , W , Vokuhl , C , Flucke , U , Petersen , I , Mechtersheimer , G , Capper , D , Jones , DT , Frohling , S , Pfister , SM & von Deimling , A 2021 , ' Sarcoma classification by DNA methylation profiling ' , Nature Communications , vol. 12 , no. 1 , 498 .
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1338020223
Document Type :
Electronic Resource