Chlorophyll a fluorescence as tool in breeding drought stress-tolerant soybean

Mitigating the effects of changing climate with adaptable cultivars while reducing the input burden of additional selection criteria is becoming a priority, especially as water shortage is limiting soybean production in Europe. To evaluate the functionality of the photosynthetic apparatus in drought, chlorophyll a (Chl a) fluorescence was measured in 16 elite soybean lines in drought and conditions with sufficient water supply in V2 (second node), R1 (beginning bloom), R4 (full pod), R5 (beginning seed) and R6 (full seed) stages. Developmental stage was a significant source of variation for all parameters, and PItotal was chosen as the most sensitive parameter in detecting the average drought effect. Genotypes G5, G9, and G10 had superior overall functioning of the photosynthetic apparatus in drought, while the photosynthetic apparatus of G12 and G16 was the least functional. The drought effect was determined to be the most relevant in R1, R4 and R6. G14 had highest PItotal in drought conditions in R1 and R4, while G9 had the highest drought-stressed PItotal in R6. G7 had the lowest drought-stressed PItotal in R1, G4 and G6 had the lowest in R4, and G8 had the lowest in R6. PItotal proved useful in breeding for abiotic stress tolerance, especially for excluding the material with the poorest photosynthetic apparatus function, which increases the efficiency of the selection process when a large number of genotypes needs to be screened. However, genotypes with superior photosynthetic apparatus functioning should be further tested in yield trials to confirm their drought tolerance and value for use in drought conditions.
Ublažavanje negativnih posljedica klimatskih promjena stvaranjem adaptabilnih sorti bez opterećenja selekcije dodatnim troškovima od velikog je značaja, osobito jer suša sve više ograničava proizvodnju soje u Europi. Za procjenu funkcionalnosti fotosintetskog aparata u suši, fluorescencija klorofila a (Chl a) mjerena je na 16 elitnih linija soje u suši i uvjetima dovoljne opskrbe vodom u V2 (drugi nodij), R1 (početak cvatnje), R4 (puni razvoj mahuna), R5 (začetak formiranja sjemena) i R6 (puni razvoj sjemena) fazama. Razvojna faza bila je značajan izvor varijacija za sve parametre, a PItotal parametar je odabran kao najosjetljiviji u otkrivanju prosječnog učinka suše. Genotipovi G5, G9 i G10 imali su superiorno funkcioniranje fotosintetskog aparata u suši, a G12 i G16 najmanje funkcionalan fotosintetski aparat u suši. Utvrđeno je da je učinak suše najrelevantniji u R1, R4 i R6 fazama razvoja. Fotosintetski aparat G14 genotipa bio je najmanje osjetljiv na sušu u R1 i R4 fazama razvoja, dok je kod G9 bio najmanje osjetljiv u R6. G7 je imao najmanje funkcionalan fotosintetski aparat u uvjetima suše u R1, G4 i G6 su imali najmanje funkcionalan fotosintetski aparata u uvjetima suše u R4, a G8 u R6. Uporaba PItotal u oplemenjivanju na tolerantnost prema abiotskom stresu pokazala se korisnom, posebice za isključenje materijala s najlošijim funkcioniranjem fotosintetskog aparata, što povećava učinkovitost selekcijskog procesa kada je potrebno procijeniti veliki broj genotipova. Međutim, genotipove sa superiornim funkcioniranjem fotosintetskog aparata potrebno je dodatno ispitati kako bi se potvrdila njihova tolerantnost i uporabna vrijednost u uvjetima suše.