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High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach
- Source :
- Mollerup , S , Asplund , M , Friis-Nielsen , J , Kjartansdóttir , K R , Fridholm , H , Hansen , T A , Herrera , J A R , Barnes , C J , Jensen , R H , Richter , S R , Nielsen , I B , Pietroni , C , Alquezar-Planas , D E , Rey-Iglesia , A , Olsen , P V S , Rajpert-De Meyts , E , Groth-Pedersen , L , von Buchwald , C , Jensen , D H , Gniadecki , R , Høgdall , E , Langhoff , J L , Pete , I , Vereczkey , I , Baranyai , Z , Dybkaer , K , Johnsen , H E , Steiniche , T , Hokland , P , Rosenberg , J , Baandrup , U , Sicheritz-Pontén , T , Willerslev , E , Brunak , S , Lund , O , Mourier , T , Vinner , L , Izarzugaza , J M G , Nielsen , L P & Hansen , A J 2019 , ' High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach ' , The Journal of Infectious Diseases , vol. 220 , no. 8 , pp. 1312-1324 .
- Publication Year :
- 2019
-
Abstract
- BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.
Details
- Database :
- OAIster
- Journal :
- Mollerup , S , Asplund , M , Friis-Nielsen , J , Kjartansdóttir , K R , Fridholm , H , Hansen , T A , Herrera , J A R , Barnes , C J , Jensen , R H , Richter , S R , Nielsen , I B , Pietroni , C , Alquezar-Planas , D E , Rey-Iglesia , A , Olsen , P V S , Rajpert-De Meyts , E , Groth-Pedersen , L , von Buchwald , C , Jensen , D H , Gniadecki , R , Høgdall , E , Langhoff , J L , Pete , I , Vereczkey , I , Baranyai , Z , Dybkaer , K , Johnsen , H E , Steiniche , T , Hokland , P , Rosenberg , J , Baandrup , U , Sicheritz-Pontén , T , Willerslev , E , Brunak , S , Lund , O , Mourier , T , Vinner , L , Izarzugaza , J M G , Nielsen , L P & Hansen , A J 2019 , ' High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach ' , The Journal of Infectious Diseases , vol. 220 , no. 8 , pp. 1312-1324 .
- Notes :
- application/pdf, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322732151
- Document Type :
- Electronic Resource