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Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients:Toward a New Approach for Diagnosis and Patient Stratification

Authors :
Poulsen, Nicklas N
Pedersen, Morten E
Østergaard, Jesper
Petersen, Nickolaj J
Nielsen, Christoffer T
Heegaard, Niels H H
Jensen, Henrik
Poulsen, Nicklas N
Pedersen, Morten E
Østergaard, Jesper
Petersen, Nickolaj J
Nielsen, Christoffer T
Heegaard, Niels H H
Jensen, Henrik
Source :
Poulsen , N N , Pedersen , M E , Østergaard , J , Petersen , N J , Nielsen , C T , Heegaard , N H H & Jensen , H 2016 , ' Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients : Toward a New Approach for Diagnosis and Patient Stratification ' , Analytical Chemistry , vol. 88 , no. 18 , pp. 9056-61 .
Publication Year :
2016

Abstract

Detection of immune responses is important in the diagnosis of many diseases. For example, the detection of circulating autoantibodies against double-stranded DNA (dsDNA) is used in the diagnosis of Systemic Lupus Erythematosus (SLE). It is, however, difficult to reach satisfactory sensitivity, specificity, and accuracy with established assays. Also, existing methodologies for quantification of autoantibodies are challenging to transfer to a point-of-care setting. Here we present the use of flow-induced dispersion analysis (FIDA) for rapid (minutes) measurement of autoantibodies against dsDNA. The assay is based on Taylor dispersion analysis (TDA) and is fully automated with the use of standard capillary electrophoresis (CE) based equipment employing fluorescence detection. It is robust toward matrix effects as demonstrated by the direct analysis of samples composed of up to 85% plasma derived from human blood samples, and it allows for flexible exchange of the DNA sequences used to probe for the autoantibodies. Plasma samples from SLE positive patients were analyzed using the new FIDA methodology as well as by standard indirect immunofluorescence and solid-phase immunoassays. Interestingly, the patient antibodies bound DNA sequences with different affinities, suggesting pronounced heterogeneity among autoantibodies produced in SLE. The FIDA based methodology is a new approach for autoantibody detection and holds promise for being used for patient stratification and monitoring of disease activity.

Details

Database :
OAIster
Journal :
Poulsen , N N , Pedersen , M E , Østergaard , J , Petersen , N J , Nielsen , C T , Heegaard , N H H & Jensen , H 2016 , ' Flow-Induced Dispersion Analysis for Probing Anti-dsDNA Antibody Binding Heterogeneity in Systemic Lupus Erythematosus Patients : Toward a New Approach for Diagnosis and Patient Stratification ' , Analytical Chemistry , vol. 88 , no. 18 , pp. 9056-61 .
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1322686906
Document Type :
Electronic Resource