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No effect of ablation of surfactant protein-D on acute cerebral infarction in mice
- Source :
- Lambertsen , K L , Østergaard , K , Clausen , B H , Hansen , S , Stenvang , J , Thorsen , S B , Meldgaard , M , Kristensen , B W , Hansen , P B L , Sørensen , G L & Finsen , B 2014 , ' No effect of ablation of surfactant protein-D on acute cerebral infarction in mice ' , Journal of Neuroinflammation , vol. 11 , 123 .
- Publication Year :
- 2014
-
Abstract
- BACKGROUND: Crosstalk between the immune system in the brain and the periphery may contribute to the long-term outcome both in experimental and clinical stroke. Although, the immune defense collectin surfactant protein-D (SP-D) is best known for its role in pulmonary innate immunity, SP-D is also known to be involved in extrapulmonary modulation of inflammation in mice. We investigated whether SP-D affected cerebral ischemic infarction and ischemia-induced inflammatory responses in mice.METHODS: The effect of SP-D was studied by comparing the size of ischemic infarction and the inflammatory and astroglial responses in SP-D knock out (KO) and wild type (WT) mice subjected to permanent middle cerebral artery occlusion. SP-D mRNA production was assessed in isolated cerebral arteries and in the whole brain by PCR, and SP-D protein in normal appearing and ischemic human brain by immunohistochemistry. Changes in plasma SP-D and TNF were assessed by ELISA and proximity ligation assay, respectively.RESULTS: Infarct volumetric analysis showed that ablation of SP-D had no effect on ischemic infarction one and five days after induction of ischemia. Further, ablation of SP-D had no effect on the ischemia-induced increase in TNF mRNA production one day after induction of ischemia; however the TNF response to the ischemic insult was affected at five days. SP-D mRNA was not detected in parenchymal brain cells in either naïve mice or in mice subjected to focal cerebral ischemia. However, SP-D mRNA was detected in middle cerebral artery cells in WT mice and SP-D protein in vascular cells both in normal appearing and ischemic human brain tissue. Measurements of the levels of SP-D and TNF in plasma in mice suggested that levels were unaffected by the ischemic insult. Microglial-leukocyte and astroglial responses were comparable in SP-D KO and WT mice.CONCLUSIONS: SP-D synthesis in middle cerebral artery cells is consistent with SP-D conceivably leaking into the
Details
- Database :
- OAIster
- Journal :
- Lambertsen , K L , Østergaard , K , Clausen , B H , Hansen , S , Stenvang , J , Thorsen , S B , Meldgaard , M , Kristensen , B W , Hansen , P B L , Sørensen , G L & Finsen , B 2014 , ' No effect of ablation of surfactant protein-D on acute cerebral infarction in mice ' , Journal of Neuroinflammation , vol. 11 , 123 .
- Notes :
- application/pdf, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322652321
- Document Type :
- Electronic Resource