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Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine
- Source :
- Barregard , L , Møller , P , Henriksen , T , Mistry , V , Koppen , G , Rossner , P , Sram , R J , Weimann , A , Poulsen , H E , Nataf , R , Andreoli , R , Manini , P , Marczylo , T , Lam , P , Evans , M D , Kasai , H , Kawai , K , Li , Y-S , Sakai , K , Singh , R , Teichert , F , Farmer , P B , Rozalski , R , Gackowski , D , Siomek , A , Saez , G T , Cerda , C , Broberg , K , Lindh , C , Hossain , M B , Haghdoost , S , Hu , C-W , Chao , M-R , Wu , K-Y , Orhan , H , Senduran , N , Smith , R J , Santella , R M , Su , Y , Cortez , C , Yeh , S , Olinski , R , Loft , S & Cooke , M S 2013 , ' Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine ' , Antioxidants & Redox Signaling , vol. 18 , no. 18 , pp. 2377-91 .
- Publication Year :
- 2013
-
Abstract
- Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is a widely used biomarker of oxidative stress. However, variability between chromatographic and ELISA methods hampers interpretation of data, and this variability may increase should urine composition differ between individuals, leading to assay interference. Furthermore, optimal urine sampling conditions are not well defined. We performed inter-laboratory comparisons of 8-oxodG measurement between mass spectrometric-, electrochemical- and ELISA-based methods, using common within-technique calibrants to analyze 8-oxodG-spiked phosphate-buffered saline and urine samples. We also investigated human subject- and sample collection-related variables, as potential sources of variability.
Details
- Database :
- OAIster
- Journal :
- Barregard , L , Møller , P , Henriksen , T , Mistry , V , Koppen , G , Rossner , P , Sram , R J , Weimann , A , Poulsen , H E , Nataf , R , Andreoli , R , Manini , P , Marczylo , T , Lam , P , Evans , M D , Kasai , H , Kawai , K , Li , Y-S , Sakai , K , Singh , R , Teichert , F , Farmer , P B , Rozalski , R , Gackowski , D , Siomek , A , Saez , G T , Cerda , C , Broberg , K , Lindh , C , Hossain , M B , Haghdoost , S , Hu , C-W , Chao , M-R , Wu , K-Y , Orhan , H , Senduran , N , Smith , R J , Santella , R M , Su , Y , Cortez , C , Yeh , S , Olinski , R , Loft , S & Cooke , M S 2013 , ' Human and methodological sources of variability in the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine ' , Antioxidants & Redox Signaling , vol. 18 , no. 18 , pp. 2377-91 .
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322643254
- Document Type :
- Electronic Resource