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Metagenomic species profiling using universal phylogenetic marker genes
- Source :
- Sunagawa , S , Mende , D R , Zeller , G , Izquierdo-Carrasco , F , Berger , S A , Kultima , J R , Coelho , L P , Arumugam , M , Tap , J , Nielsen , H B , Rasmussen , S , Brunak , S , Pedersen , O B , Guarner , F , de Vos , W M , Wang , J , Li , J , Doré , J , Ehrlich , S D , Stamatakis , A & Bork , P 2013 , ' Metagenomic species profiling using universal phylogenetic marker genes ' , Nature Methods , vol. 10 , no. 12 , pp. 1196-1199 .
- Publication Year :
- 2013
-
Abstract
- To quantify known and unknown microorganisms at species-level resolution using shotgun sequencing data, we developed a method that establishes metagenomic operational taxonomic units (mOTUs) based on single-copy phylogenetic marker genes. Applied to 252 human fecal samples, the method revealed that on average 43% of the species abundance and 58% of the richness cannot be captured by current reference genome-based methods. An implementation of the method is available at http://www.bork.embl.de/software/mOTU/.
Details
- Database :
- OAIster
- Journal :
- Sunagawa , S , Mende , D R , Zeller , G , Izquierdo-Carrasco , F , Berger , S A , Kultima , J R , Coelho , L P , Arumugam , M , Tap , J , Nielsen , H B , Rasmussen , S , Brunak , S , Pedersen , O B , Guarner , F , de Vos , W M , Wang , J , Li , J , Doré , J , Ehrlich , S D , Stamatakis , A & Bork , P 2013 , ' Metagenomic species profiling using universal phylogenetic marker genes ' , Nature Methods , vol. 10 , no. 12 , pp. 1196-1199 .
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322641380
- Document Type :
- Electronic Resource