Back to Search
Start Over
Self-peptides with intermediate capacity to bind and stabilize MHC class I molecules may be immunogenic.
- Source :
- Andersen , M L M , Ruhwald , M , Nissen , M H , Buus , S & Claesson , M H 2003 , ' Self-peptides with intermediate capacity to bind and stabilize MHC class I molecules may be immunogenic. ' , Scandinavian Journal of Immunology , vol. 57 , no. 1 , pp. 21-7 .
- Publication Year :
- 2003
-
Abstract
- Udgivelsesdato: 2003-Jan<br />Thirty self-peptides were selected on the basis of their predicted binding to H-2b molecules. The binding of peptides was ascertained experimentally by biochemical (KD measurements) and cellular [major histocompatibility complex class I (MHC-I) stabilization] assays. A weak, but significant, correlation between KD measurements and MHC-I stabilization was observed. Mice (n = 99) were immunized with individual peptides. Twenty-eight peptides were found to induce peptide-specific cytotoxic activity, and a total of 84 mice developed significant cytotoxic T lymphocyte (CTL) responses after immunization. Only one of the 21 mice immunized with high-affinity peptides developed a peptide-specific CTL response of 29 lytic units per 106 splenocytes, whereas 11 of the 42 mice immunized with intermediate-affinity peptides developed peptide-specific CTL responses at this level (P < 0.05). These observations suggest the absence of tolerance towards most MHC-I-restricted self-peptides and that strong antiself immunity can be generated preferentially towards self-peptides with an intermediate affinity for MHC-I. These data should be considered in the design of tumour vaccines based on MHC-I-binding self-peptides.
Details
- Database :
- OAIster
- Journal :
- Andersen , M L M , Ruhwald , M , Nissen , M H , Buus , S & Claesson , M H 2003 , ' Self-peptides with intermediate capacity to bind and stabilize MHC class I molecules may be immunogenic. ' , Scandinavian Journal of Immunology , vol. 57 , no. 1 , pp. 21-7 .
- Notes :
- English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1322567419
- Document Type :
- Electronic Resource