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NMR analysis of budding yeast metabolomics: A rapid method for sample preparation

Authors :
Airoldi, C
Tripodi, F
Guzzi, C
Nicastro, R
Coccetti, P
AIROLDI, CRISTINA
TRIPODI, FARIDA
GUZZI, CINZIA
NICASTRO, RAFFAELE
COCCETTI, PAOLA
Airoldi, C
Tripodi, F
Guzzi, C
Nicastro, R
Coccetti, P
AIROLDI, CRISTINA
TRIPODI, FARIDA
GUZZI, CINZIA
NICASTRO, RAFFAELE
COCCETTI, PAOLA
Publication Year :
2015

Abstract

Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.

Details

Database :
OAIster
Notes :
STAMPA, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1308911845
Document Type :
Electronic Resource