Trigona Honey, a Natural Bee Product Promotes mRNA Foxp3 Expression in Healthy in Mice Balb/c Strain

The highest Foxp3 mRNA expression in Trigona honey was the group given with a dose of 0.27 ml/20 g Bw (p=0.000, however, the group given Trigona Honey with a dose of 0.23 ml/20 g Bw also had moderate Foxp3 mRNA expression. These data suggested that Trigona honey could induce Foxp3mRNAexpression. The higher dose given, the higher Foxp3 mRNA expression. Transcription factor, Foxp3 Treg plays an important role in the balance of the immune system, diets containing polyphenols and flavonoids could increase the expression of FOXP3mRNA although some studies have contrary results. Trigona honey is a specific honey from Trigona bees containing polyphenols and could influence the immune homeostasis. There have never beenstudies provingits effectson the expression ofFoxp3mRNA as the transcription factor of Regulatory TCell. It was a laboratory research, mice Balb/c were divided into the reference, positive and treatment groups. The reference group was only given standard feed and positive control was intraperitonially injected with Salmonella Enterica serovarTyphi. The treatment group was divided into 2 groups and given Trigona honey using canule with both doses of 0.23 ml/20 g Bw and 0.27 ml/20 g Bw daily for 10 days respectively. Foxp3 mRNA expression was examined by real-time RT-PCR. Repeated Anova and One Way Anova were used as the statistical methods, a p-value of less than 0.050 at the final analysis was considered indicating statistical significance. Results indicated Foxp3mRNAexpression of the groups given by honey was higher than the control group.