Jiangella anatolica sp. nov. isolated from coastal lake soil

A novel actinobacterial strain, designatedGTF31T, was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia,Turkey. The taxonomic position of the strain wasestablished using a polyphasic approach. Phylogeneticanalyses based on 16S rRNA gene sequences andshowed that the strain is closely related toJiangellagansuensisDSM 44835T(99.4%),Jiangella alba DSM 237T(99.3%) andJiangella muralisDSM45357T(99.2%). Optimal growth was observed at 28°C and pH 7–8. Whole cell hydrolysates werefound to contain LL-DAP, glucose, mannose, rham-nose and ribose. The predominant menaquinone wasidentified as MK-9(H4). The polar lipid profile wasfound to contain diphosphatidylglycerol, phos-phatidylglycerol, phosphatidylinositol, glycophospho-lipids and unidentified phospholipids. The major fattyacids were identified as anteiso-C15:0, iso-C16:0andanteiso-C17:0. The content of the type strainwas determined to be 72.5% and the size of the draftgenome is 7.0 Mb. The calculated digital DDH valuesbetween strain GTF31Tand the type strains ofJ.gansuensis,J. alba,J. muralisandJiangella alka-liphilaranged from 24.4 to 34.4% and ANI values ranged between 81.0 and 87.9%. Based upon theconsensus of phenotypic and phylogenetic analyses aswell as whole genome comparisons, strain GTF31T(= DSM 100984T= CECT 9378T) is proposed torepresent the type strain of a novel species,Jiangellaanatolicasp. nov