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Fully integrated Monte Carlo simulation for evaluating radiation induced DNA damage and subsequent repair using Geant4-DNA

Authors :
Sakata, Dousatsu
Belov, Oleg
Bordage, Marie-Claude
Emfietzoglou, Dimitris
Guatelli, Susanna
Inaniwa, Taku
Ivanchenko, Vladimir
Karamitros, Mathieu
Kyriakou, Ioanna
Lampe, Nathanael
Petrović, Ivan M.
Ristić-Fira, Aleksandra
Shin, Wook-Geun
Incerti, Sebastien
Sakata, Dousatsu
Belov, Oleg
Bordage, Marie-Claude
Emfietzoglou, Dimitris
Guatelli, Susanna
Inaniwa, Taku
Ivanchenko, Vladimir
Karamitros, Mathieu
Kyriakou, Ioanna
Lampe, Nathanael
Petrović, Ivan M.
Ristić-Fira, Aleksandra
Shin, Wook-Geun
Incerti, Sebastien
Source :
Scientific Reports
Publication Year :
2020

Abstract

Ionising radiation induced DNA damage and subsequent biological responses to it depend on the radiation's track-structure and its energy loss distribution pattern. To investigate the underlying biological mechanisms involved in such complex system, there is need of predicting biological response by integrated Monte Carlo (MC) simulations across physics, chemistry and biology. Hence, in this work, we have developed an application using the open source Geant4-DNA toolkit to propose a realistic "fully integrated" MC simulation to calculate both early DNA damage and subsequent biological responses with time. We had previously developed an application allowing simulations of radiation induced early DNA damage on a naked cell nucleus model. In the new version presented in this work, we have developed three additional important features: (1) modeling of a realistic cell geometry, (2) inclusion of a biological repair model, (3) refinement of DNA damage parameters for direct damage and indirect damage scoring. The simulation results are validated with experimental data in terms of Single Strand Break (SSB) yields for plasmid and Double Strand Break (DSB) yields for plasmid/human cell. In addition, the yields of indirect DSBs are compatible with the experimental scavengeable damage fraction. The simulation application also demonstrates agreement with experimental data of gamma -H2AX yields for gamma ray irradiation. Using this application, it is now possible to predict biological response along time through track-structure MC simulations.

Details

Database :
OAIster
Journal :
Scientific Reports
Notes :
Scientific Reports
Publication Type :
Electronic Resource
Accession number :
edsoai.on1286028474
Document Type :
Electronic Resource