Enzymatic mineralization of hydrogels for bone tissue engineering by incorporation of alkaline phosphatase.

Authors :: Douglas, T.E.L.
Messersmith, P.B.
Chasan, S.
Mikos, A.G.
Mulder, E.L.W. de
Dickson, G.
Schaubroeck, D.
Balcaen, L.
Vanhaecke, F.
Dubruel, P.
Jansen, J.A.
Leeuwenburgh, S.C.G.
Douglas, T.E.L.
Messersmith, P.B.
Chasan, S.
Mikos, A.G.
Mulder, E.L.W. de
Dickson, G.
Schaubroeck, D.
Balcaen, L.
Vanhaecke, F.
Dubruel, P.
Jansen, J.A.
Leeuwenburgh, S.C.G.
Source :: Macromolecular Bioscience; 1077; 1089; 1616-5187; 8; 12; ~Macromolecular Bioscience~1077~1089~~~1616-5187~8~12~~
Publication Year :: 2012
Abstract: 1 augustus 2012<br />Item does not contain fulltext<br />Alkaline phosphatase (ALP), an enzyme involved in mineralization of bone, is incorporated into three hydrogel biomaterials to induce their mineralization with calcium phosphate (CaP). These are collagen type I, a mussel-protein-inspired adhesive consisting of PEG substituted with catechol groups, cPEG, and the PEG/fumaric acid copolymer OPF. After incubation in Ca-GP solution, FTIR, EDS, SEM, XRD, SAED, ICP-OES, and von Kossa staining confirm CaP formation. The amount of mineral formed decreases in the order cPEG > collagen > OPF. The mineral:polymer ratio decreases in the order collagen > cPEG > OPF. Mineralization increases Young's modulus, most profoundly for cPEG. Such enzymatically mineralized hydrogel/CaP composites may find application as bone regeneration materials.

Database :: OAIster
Journal :: Macromolecular Bioscience; 1077; 1089; 1616-5187; 8; 12; ~Macromolecular Bioscience~1077~1089~~~1616-5187~8~12~~
Publication Type :: Electronic Resource
Accession number :: edsoai.on1284045262
Document Type :: Electronic Resource

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