LncRNA SAMMSON Overexpression Suppresses Vascular Smooth Muscle Cell Proliferation via Inhibiting miR-130a Maturation to Participate in Intracranial Aneurysm

Wen Pan,1 Yuan Gao,2 Weifeng Wan,3 Wenfeng Xiao,4 Chao You5 1Brain Center, Shougang Shuigang General Hospital, Liupanshui City, Guizhou Province, 553000, People’s Republic of China; 2Department of Neurosurgery, Liupanshui People’s Hospital, Liupanshui City, Guizhou Province, 553001, People’s Republic of China; 3Department of Neurosurgery, Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province, 646000, People’s Republic of China; 4Department of Neurosurgery, Sichuan Mianyang 404 Hospital, Mianyang City, Sichuan Province, 510700, People’s Republic of China; 5Department of Neurosurgery West China Hospital, Sichuan University, Chengdu Province, 610041, People’s Republic of ChinaCorrespondence: Chao YouDepartment of Neurosurgery West China Hospital, Sichuan University, 37 Guoxue Lane, Wuhou District, Chengdu Province, 610041, People’s Republic of ChinaEmail chaoyousichuan@163.comBackground: MiR-130a is a recently identified critical player in vascular smooth muscle cell (VSMC) proliferation, which participates in intracranial aneurysm (IA). However, the involvement of miR-130a in IA and its upstream regulator are unknown. Our preliminary sequencing analysis revealed a close correlation between miR-130a and lncRNA SAMMSON across IA samples. Therefore, we further studied the crosstalk between SAMMSON and miR-130a in IA.Methods: SAMMSON and miR-130a expression were measured using RT-qPCR. SAMMSON subcellular location was analyzed with nuclear fractionation assay. Their direct interaction was explored with RNA pull-down assay. The role of SAMMSON in miR-130a maturation was studied with overexpression analysis. VSMC cell proliferation was analyzed with BrdU assay.Results: SAMMSON and premature miR-130a were deregulated in IA, while mature miR-130a was upregulated in IA. SAMMSON is localized in both the nucleus and cytoplasm, and direct interaction between SAMMSON and miR-130a was