Rapid identification of phytopathogenic bacteria originating from vegetables by the BIOLOG System

The purpose of our study was to investigate the possibility of using the BIOLOG System for a rapid identification of various phytopathogenic bacteria (all in all 19 strains) from different vegetable crops. The results of the study have shown that the BIOLOG System indeed enables a rapid identification of numerous strains of phytopathogenic bacteria belonging to the species Pseudomonas syringae, Xanthomonas campestris and Erwinia carotovora. After four, six or twenty - four hours of incubation, identification down to the level of pathovar (Ps.pv. phaseolicola, P.s.pv. syringae, P.s.pv. tomato, X.c.pv. phaseoli and Xc.pv. vesicatoria) or subspecies (E.c.ssp. carotovora and E.c.ssp. atroseptica) was possible for 14 out of 19 investigated strains. For the others, the identification proved correct at the level of species or genus. Some pathovars were wrongly identified.