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A comprehensive enhancer screen identifies TRAM2 as a key and novel mediator of YAP oncogenesis
- Publication Year :
- 2021
-
Abstract
- Background: Frequent activation of the co-transcriptional factor YAP is observed in a large number of solid tumors. Activated YAP associates with enhancer loci via TEAD4-DNA-binding protein and stimulates cancer aggressiveness. Although thousands of YAP/TEAD4 binding-sites are annotated, their functional importance is unknown. Here, we aim at further identification of enhancer elements that are required for YAP functions. Results: We first apply genome-wide ChIP profiling of YAP to systematically identify enhancers that are bound by YAP/TEAD4. Next, we implement a genetic approach to uncover functions of YAP/TEAD4-associated enhancers, demonstrate its robustness, and use it to reveal a network of enhancers required for YAP-mediated proliferation. We focus on EnhancerTRAM2, as its target gene TRAM2 shows the strongest expression-correlation with YAP activity in nearly all tumor types. Interestingly, TRAM2 phenocopie
Details
- Database :
- OAIster
- Notes :
- application/pdf, Genome Biology vol. 22 no. 1, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1244879666
- Document Type :
- Electronic Resource
- Full Text :
- https://doi.org/10.1186.s13059-021-02272-8