Uticaj ishrane obogaćene OMEGA-3 i OMEGA-6 masnim kiselinama na funkciju miokarda i oksidativno-inflamacijske parametre kod srca starih pacova

Uvod: Rezultati istraživanja daju originalan i važan doprinos sagledavanju pozitivnih i nagativnih efekata polinezasićenih masnih kiselina zastupljenih u ishrani, kao i mogućnostima i potrebama izmene njihovog odnosa u cilju postizanja povoljnih efekata na kardiovaskularni sisteam. Rezultati istraživanja imaju veliki praktični značaj, pre svega imajući u vidu da se radi o ispitivanju organskog sistema čiji poremećaji zahvataju daleko najveći postotak svetske populacije. Cilj: Cilj ovog istraživanja je bilo ispitivanje hroničnih efekata primene ishrane obogaćene omega-3 i omega-6 masnim kiselinama na funkciju miokarda, koronarni protok i oksido-inflamacijske parametre starih pacova. Materijal i metode: Istraživanje je dizajnirano kao eksperimentalna studija sprovedena ex vivo. Nakon hroničnog tretmana od 6 nedelja odgovarajućom ishranom, životinje su se žrtvovale kako bi se sprovela istraživanja na izolovanom srcu i biohemijske analize iz uzoraka perfuzata, plazme i lizata eritrocita. Biohemijske analize su određivane u uzorcima koronarnog venskog efluenta, koji je sakupljan na kraju kontrolnog perioda i na kraju perioda aplikacije ispitivane supstance. Svi reaktivni molekuli, koji su bili od interesa za naše istraživanje su mereni spektrofotometrijskom metodom. Pored toga, u uzorcima plazme i lizatu eritrocita merile su se vrednosti pro-oksidacionih, antioksidacionih i inflamatornih parametara. Rezultati: Hronična primena suplementacije alfa-linoleinskom kiselinom ima kardioprotektivno dejstvo u odnosu na primenu linolne kiseline na modelu izolovanog srca pacova. Ishrana obogaćena omega-6 masnim kiselinama utiče negativno na funkciju miokarda izolovanog srca pacova u odnosu na ishranu obogaćenu omega-3 masnim kiselinama. Primenom hronične ishrane sa odnosom omega-3/omega-6 u našem istraživanju 1:3,3 (55%:17%) u korist omega-3 masnih kiselina u vidu ulja lanenog semena, kao i primena masnih kiselina u odnosu omega-3/omega-6 1:9 (9%:73%) u vidu ulja noćurka u korist o
Introduction: The results of the research give an original and important contribution to the examination of the positive and negative effects of polyunsaturated fatty acids present in the diet, as well as the possibilities and needs of changing their relationship in order to achieve favorable effects on the cardiovascular system. The results of the research have a great practical significance, predominantly having in mind that this disruptions of this organic system are by far the largest percentage of the world population. Aim: The aim of this study was to examine the chronic effects of diet enriched with omega-3 and omega-6 fatty acids on myocardial function, coronary flow and oxidizing inflammatory parameters of old rats. Material and methods: The study was designed as an ex vivo experimental study. After 6 weeks of chronic treatment with adequate nutrition, animals were sacrificed to perform isolated heart investigation and biochemical analysis from perfusate, plasma, and erythrocyte lysates. Biochemical analyzes were determined in samples of the coronary venous effluent, which was collected at the end of the control period and at the end of the period of application of the tested substance. All reactive molecules that were of interest to our research were measured by a spectrophotometric method. In addition, in the plasma and lysate erythrocyte samples, the values of pro-oxidation, antioxidant and inflammatory parameters were measured. Results: The alpha-linoleic acid supplementation has a stronger cardioprotective effect than linoleic acid on the model of the isolated rat heart. Nutrition enriched with omega-6 fatty acids more affects the myocardial function of the isolated heart of the rat in relation to than diet enriched with omega-3 fatty acids. Using chronic nutrition with omega-3 / omega-6 ratio 1:3.3 (55%: 17%) in favor of omega-3 fatty acids in the form of flax seed oil, and the application of fatty acids in the ratio of omega-3 / omega-6 1:9 (95:73%)