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SLC20A1Is Involved in Urinary Tract and Urorectal Development

Authors :
Rieke, Johanna Magdalena
Zhang, Rong
Braun, Doreen
Yilmaz, Oeznur
Japp, Anna S.
Lopes, Filipa M.
Pleschka, Michael
Hilger, Alina C.
Schneider, Sophia
Newman, William G.
Beaman, Glenda M.
Nordenskjoeld, Agneta
Ebert, Anne-Karoline
Promm, Martin
Roesch, Wolfgang H.
Stein, Raimund
Hirsch, Karin
Schaefer, Frank-Mattias
Schmiedeke, Eberhard
Boemers, Thomas M.
Lacher, Martin
Kluth, Dietrich
Gosemann, Jan-Hendrik
Anderberg, Magnus
Barker, Gillian
Holmdahl, Gundela
Läckgren, Göran
Keene, David
Cervellione, Raimondo M.
Giorgio, Elisa
Di Grazia, Massimo
Feitz, Wouter F. J.
Marcelis, Carlo L. M.
Van Rooij, Iris A. L. M.
Boekenkamp, Arend
Beckers, Goedele M. A.
Keegan, Catherine E.
Sharma, Amit
Dakal, Tikam Chand
Wittler, Lars
Grote, Phillip
Zwink, Nadine
Jenetzky, Ekkehart
Brusco, Alfredo
Thiele, Holger
Ludwig, Michael
Schweizer, Ulrich
Woolf, Adrian S.
Odermatt, Benjamin
Reutter, Heiko
Rieke, Johanna Magdalena
Zhang, Rong
Braun, Doreen
Yilmaz, Oeznur
Japp, Anna S.
Lopes, Filipa M.
Pleschka, Michael
Hilger, Alina C.
Schneider, Sophia
Newman, William G.
Beaman, Glenda M.
Nordenskjoeld, Agneta
Ebert, Anne-Karoline
Promm, Martin
Roesch, Wolfgang H.
Stein, Raimund
Hirsch, Karin
Schaefer, Frank-Mattias
Schmiedeke, Eberhard
Boemers, Thomas M.
Lacher, Martin
Kluth, Dietrich
Gosemann, Jan-Hendrik
Anderberg, Magnus
Barker, Gillian
Holmdahl, Gundela
Läckgren, Göran
Keene, David
Cervellione, Raimondo M.
Giorgio, Elisa
Di Grazia, Massimo
Feitz, Wouter F. J.
Marcelis, Carlo L. M.
Van Rooij, Iris A. L. M.
Boekenkamp, Arend
Beckers, Goedele M. A.
Keegan, Catherine E.
Sharma, Amit
Dakal, Tikam Chand
Wittler, Lars
Grote, Phillip
Zwink, Nadine
Jenetzky, Ekkehart
Brusco, Alfredo
Thiele, Holger
Ludwig, Michael
Schweizer, Ulrich
Woolf, Adrian S.
Odermatt, Benjamin
Reutter, Heiko
Publication Year :
2020

Abstract

Previous studies in developingXenopusand zebrafish reported that the phosphate transporterslc20a1ais expressed in pronephric kidneys. The recent identification ofSLC20A1as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role ofSLC20A1in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish orthologslc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed immunohistochemistry of an unaffected 6-week-old human embryo and detectedSLC20A1in the urinary tract and the abdominal midline, structures implicated in the pathogenesis of cloacal exstrophy. Additionally, we resequencedSLC20A1in 690 individuals with bladder exstrophy-epispadias complex (BEEC) including 84 individuals with cloacal exstrophy. We identified two additional monoallelicde novovariants. One was identified in a case-parent trio with classic bladder exstrophy, and one additional novelde novovariant was detected in an affected mother who transmitted this variant to her affected son. To study the potential cellular impact ofSLC20A1variants, we expressed them in HEK293 cells. Here, phosphate transport was not compromised, suggesting that it is not a disease mechanism. However, there was a tendency for lower levels of cleaved caspase-3, perhaps implicating apoptosis pathways in the disease. Our results suggestSLC20A1is involved in urinary tract and urorectal development and implicateSLC20A1as a disease-gene for BEEC.

Details

Database :
OAIster
Notes :
application/pdf, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1234689340
Document Type :
Electronic Resource
Full Text :
https://doi.org/10.3389.fcell.2020.00567