Perfusion of an IgG producing CHO cell line at very high cell density by ATF or by TFF in WAVE Bioreactor™

Perfusion of an IgG producing CHO cell line was performed in a WAVE Bioreactor™ using either Alternating Tangential Flow or Tangential Flow Filtration. The properties and performances obtained in this bioreactor with both filtration systems were studied. Very high cell densities were achieved and could be stably maintained at high viability indicating of a healthy process suitable for instance for efficient cell banking. Cell density could be significantly further increased showing the capacity of the system set-up.
Invited speaker at the Hanson Wade Conference Optimizing Cell Line Performance, July, 26-28, 2011, London, UK. QC 20130319