Freezing stallion semen with the new Caceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability

Authors :: Morillo Rodriguez, A
Ortega Ferrusola, C
Macias Garcia, B
Morrell, J M
Rodriguez-Martinez, Heriberto
Tapia, J A
Pena, F J
Morillo Rodriguez, A
Ortega Ferrusola, C
Macias Garcia, B
Morrell, J M
Rodriguez-Martinez, Heriberto
Tapia, J A
Pena, F J
Publication Year :: 2011
Abstract: Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytometry). Samples frozen in Caceres extender consistently showed the best results in post-thaw motility (increases ranging from 11 to 17%, p andlt; 0.05) and velocity (p andlt; 0.05), membrane integrity (increases ranging from 11 to 14%, p andlt; 0.05) and mitochondrial membrane potential (p andlt; 0.05). It is concluded that this new extender should be included in a freezeability test to determine the best extender for each individual.<br />Funding Agencies|Ministerio de Ciencia e Innovacion, Madrid, Spain|INIA R22008-00018-00-00AGL 2010-20758 GAN|Junta de Extremadura FEDER|GR 10010

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