Intraneural Application of microRNA-1 Mimetic Nucleotides Does Not Resolve Neuropathic Pain After Chronic Constriction Injury in Rats

Anne Kuebart,1,* Verena Wollborn,1,* Ragnar Huhn,1 Henning Hermanns,2 Robert Werdehausen,3 Timo Brandenburger1 1Department of Anesthesiology, University Hospital Düsseldorf, Medical Faculty, Düsseldorf 40225, Germany; 2Department of Anesthesiology, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands; 3Department of Anesthesiology and Intensive Care, University of Leipzig, Medical Faculty, Leipzig 04103, Germany*These authors contributed equally to this workCorrespondence: Timo BrandenburgerDepartment of Anesthesiology, University Hospital Düsseldorf, Moorenstr. 5, Düsseldorf 40225, GermanyEmail timo.brandenburger@med.uni-duesseldorf.deRobert WerdehausenDepartment of Anesthesiology and Intensive Care, University of Leipzig, Medical Faculty, Leipzig 04103, GermanyEmail robert.werdehausen@medizin.uni-leipzig.deBackground: Alterations of the expression of microRNAs (miRNAs) in chronic pain models seem to play a crucial role in the development of neuropathic pain, with microRNA-1 (miR-1) being of particular interest. Recently, we were able to show that decreased miR-1 levels were associated with increased expression of brain-derived neurotrophic factor (BDNF) and Connexin 43 (Cx43). We hypothesized that miR-1 mimetic nucleotides could alleviate neuropathic pain caused by chronic constriction injury in rats.Methods: MiR-1 mimetic nucleotides were evaluated for effectiveness, functionality, and intracellular stability by transfecting human glioblastoma cells (U-87 MG). In vivo transfection with miR-1 mimics and corresponding scrambled miRNAs serving as control was performed by repetitive injection (days 0, 3, and 7) into the sciatic nerve following chronic constriction injury (CCI) in rats. Quantitative PCR was used to measure miR-1 content. Cx43 expression was determined by Western blot analysis. Effects on neuropathic pain were assessed by detecting paw withdrawal thresholds using an automated filament application.Result