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Molecular determinants for the activation/inhibition of bak protein by BH3 peptides

Authors :
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química
Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
Vila Julià, Guillem
Granadino, Jose Manuel
Pérez González, Juan Jesús
Rubio Martínez, Jaime
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química
Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
Vila Julià, Guillem
Granadino, Jose Manuel
Pérez González, Juan Jesús
Rubio Martínez, Jaime
Publication Year :
2020

Abstract

Apoptosis is a key cell death pathway in mammalian cells. Understanding this process and its regulation has been a subject of study in the last three decades. Members of the Bcl-2 family of proteins are involved in the regulation of apoptosis through mitochondrial poration with the subsequent initiation of apoptosis. Deregulation of proapoptotic proteins contributes to the progression of many tumor processes. Understanding how these pore-forming Bcl-2 proteins Bak and Bax are activated is key to find new anticancer treatments. As no drug capable of activating Bak has been disclosed yet, the study of the structural features of BH3 peptides-known as Bak activators-relevant for binding along with its binding energy decomposition analysis, becomes essential for designing novel small-molecule mimics of BH3. Interestingly, a BH3 Bim analogue-inactivating Bak has recently been discovered, opening a question on the molecular features that determine the functions of BH3 peptides. Therefore, the present work is aimed at understanding the way BH3 peptides activate or inactivate Bak in order to identify differential structural features that can be used in drug design. For this purpose, complexes of Bak with an activator and an inhibitor have been subjected to a molecular dynamics study. Structural differences were assessed by means of the fluctuations of the corresponding principal components. Moreover, the MMPB/GBSA approach was used to compute the binding free energy of the diverse complexes to identify those residues of the BH3 peptide that exhibit the larger contributions to complex formation. The results obtained in this work show differences between activators and inhibitors, both in structural and energetic terms, which can be used in the design of new molecules that can activate or inactivate proapoptotic Bak<br />Peer Reviewed<br />Postprint (author's final draft)

Details

Database :
OAIster
Notes :
12 p., application/vnd.openxmlformats-officedocument.wordprocessingml.document, English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1159676378
Document Type :
Electronic Resource