LINC01272 Promotes Migration and Invasion of Gastric Cancer Cells via EMT

Xue Leng,1 Geli Liu,1 Sen Wang,2 Jing Song,3 Wanfeng Zhang,3 Xianqin Zhang,1 Li Rong,1,4 Yongping Ma,1 Fangzhou Song1 1Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing 400016, People’s Republic of China; 2Laboratory Department, Affiliated Hospital of Jining Medical College, Shandong, 272029, People’s Republic of China; 3Department of Bioinformatics, The Basic Medical School of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 4Department of Gastroenterology, Chongqing Public Health Medical Center, Chongqing, 400036, People’s Republic of ChinaCorrespondence: Fangzhou SongMolecular Medicine and Cancer Research Center, Chongqing Medical University, Yixueyuan Road, Yuzhong District, Chongqing 400016, People’s Republic of ChinaTel +86 23 68485958Email fzsongcq@163.comPurpose: Gastric cancer (GC) is the fifth most common tumor in the world, and most patients with GC have a poor prognosis. This study aimed to explore the biological influence and mechanism of LINC01272 in GC.Materials and Methods: Using bioinformatic analyses, we investigated the expression of LINC01272 in TCGA database and predicted the biological functions and mechanism of LINC01272 in GC. Then, we detected the expression of LINC01272 in GC cell lines, GC tissues, and corresponding normal tissues using real-time polymerase chain reaction (RT-PCR). Finally, we explored the migration and invasion ability of LINC01272 by wound-healing and Transwell assays and examined the expression of epithelial-mesenchymal transition (EMT)-related proteins through Western blotting.Results: We found that LINC01272 was upregulated in GC and was associated with GC staging and lymph node metastasis. The results of wound-healing and Transwell assays revealed that the LINC01272 was closely related to GC cell migration and invasion. LINC01272 knockdown inhibited the migration and invasion ability of GC cells