Neutrophil Responses to Sterile Implant Materials

In vivo implantation of sterile materials and devices results in a foreign body immune response leading to fibrosis of implanted material. Neutrophils, one of the first immune cells to be recruited to implantation sites, have been suggested to contribute to the establishment of the inflammatory microenvironment that initiates the fibrotic response. However, the precise numbers and roles of neutrophils in response to implanted devices remains unclear. Using a mouse model of peritoneal microcapsule implantation, we show 30–500 fold increased neutrophil presence in the peritoneal exudates in response to implants. We demonstrate that these neutrophils secrete increased amounts of a variety of inflammatory cytokines and chemokines. Further, we observe that they participate in the foreign body response through the formation of neutrophil extracellular traps (NETs) on implant surfaces. Our results provide new insight into neutrophil function during a foreign body response to peritoneal implants which has implications for the development of biologically compatible medical devices.
Mazumdar-Shaw International Oncology Fellowship
Juvenile Diabetes Research Foundation International (Grant 17-2007-1063)
Leona M. and Harry B. Helmsley Charitable Trust (Grant 09PG-T1D027)
National Institutes of Health (U.S.) (Grant EB000244)
National Institutes of Health (U.S.) (Grant EB000351)
National Institutes of Health (U.S.) (Grant DE013023)
National Institutes of Health (U.S.) (Grant CA151884)