Potential Prognostic and Diagnostic Values of CDC6, CDC45, ORC6 and SNHG7 in Colorectal Cancer

Yang Hu,1–4,* Liping Wang,5,* Zhixing Li,1–4 Zirui Wan,6 Mingjie Shao,4,7 Shaobin Wu,4,7 Guo Wang1–4 1Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China; 2Institute of Clinical Pharmacology, Central South University, Hunan Key Laboratory of Pharmacogenetics, Changsha 410078, People’s Republic of China; 3Engineering Research Center of Applied Technology of Pharmacogenomics, Ministry of Education, Changsha 410078, People’s Republic of China; 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, Hunan, People’s Republic of China; 5Department of Clinical Oncology, The First People’s Hospital of Chenzhou, Chenzhou 432000, Hunan, People’s Republic of China; 6Department of Pharmacy, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, People’s Republic of China; 7Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, Changsha 410008, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shaobin Wu; Mingjie Shao Email shaobinwu@csu.edu.cn; 148302041@csu.edu.cnBackground: Colorectal cancer (CRC) is a common human malignancy. The aims of this study are to investigate the gene expression profile of CRC and to explore potential strategy for CRC diagnosis, therapy and prognosis.Methods: We use affy and Limma package of Bioconductor R to do differential expression genes (DEGs) and differential expression lncRNAs (DELs) analysis from the gene datasets (GSE8671, GSE21510, GSE32323, GSE39582 and TCGA) respectively. Then, DEGs were analyzed by GO and KEGG pathway and Kaplan-Meier survival curve and Cox regression analyses were used to find aberrantly expressed genes associated with survival outcome of CRC patients. Real-time PCR assay was used to verify th