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Modular actin nano-architecture enables podosome protrusion and mechanosensing
- Publication Year :
- 2019
-
Abstract
- Basement membrane transmigration during embryonal development, tissue homeostasis and tumor invasion relies on invadosomes, a collective term for invadopodia and podosomes. An adequate structural framework for this process is still missing. Here, we reveal the modular actin nano-architecture that enables podosome protrusion and mechanosensing. The podosome protrusive core contains a central branched actin module encased by a linear actin module, each harboring specific actin interactors and actin isoforms. From the core, two actin modules radiate: ventral filaments bound by vinculin and connected to the plasma membrane and dorsal interpodosomal filaments crosslinked by myosin IIA. On stiff substrates, the actin modules mediate long-range substrate exploration, associated with degradative behavior. On compliant substrates, the vinculin-bound ventral actin filaments shorten, resulting in short-range connectivity and a focally protrusive, non-degradative state. Our findings redefine podosome nanoscale architecture and reveal a paradigm for how actin modularity drives invadosome mechanosensing in cells that breach tissue boundaries.
Details
- Database :
- OAIster
- Notes :
- application/pdf, Nature Communications vol. 10 no. 1, English
- Publication Type :
- Electronic Resource
- Accession number :
- edsoai.on1134975493
- Document Type :
- Electronic Resource
- Full Text :
- https://doi.org/10.1038.s41467-019-13123-3