Effect of hyperthemia induced by Nd:YAG laser and photodynamic therapy by Ar+ laser radiation in combination on the transplanted tumor in CM mice

The present paper reports the effect of hyperthermia (HT) induced by Nd:YAG laser and photodynamic therapy (PDT) by AR + laser radiation in combination on the transplanted sarcoma 180 in CM mice. Tumor-bearing mice were divided into four groups: A) control group, B) HT group, C) PDT group and D) PDT followed by HT group. HT was induced by Nd:YAG laser radiation and the temperature at tumor edge was control at 43 degrees C for 30 minutes. Tumor-bearing mice of group C and D were given an i.p. injection of HpD 24 hours prior to AR + laser radiation. The experiments show significant increases in inhibition rates against the S-180 in CM mice by the HT and PDT combination, compared to those by either HT or PDT alone. With that of Group D being the lowest, the DNA contents of tumor cells in the four groups are of statistical significance too. These results suggest the combination of HT induced by Nd:YAG and PDT by Ar + laser radiation may become a new method of tumor treatment.