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Alternative Formation of Red-Shifted Channelrhodopsins: Noncovalent Incorporation with Retinal-Based Enamine-Type Schiff Bases and Mutated Channelopsin

Authors :
50378535
80263200
Okitsu, Takashi
Matsuyama, Take
Yamashita, Takahiro
Ishizuka, Toru
Yawo, Hiromu
Imamoto, Yasushi
Shichida, Yoshinori
Wada, Akimori
50378535
80263200
Okitsu, Takashi
Matsuyama, Take
Yamashita, Takahiro
Ishizuka, Toru
Yawo, Hiromu
Imamoto, Yasushi
Shichida, Yoshinori
Wada, Akimori
Publication Year :
2017

Abstract

Red-shifted channelrhodopsins (ChRs) are attractive for optogenetic tools. We developed a new type of red-shifted ChRs that utilized noncovalent incorporation of retinal and 3, 4-dehydroretinal-based enamine-type Schiff bases and mutated channelopsin, C1C2-K296G. These ChRs exhibited absorption maxima that were shifted 10–30 nm toward longer wavelengths than that of C1C2-ChR regenerated with all-trans-retinal.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1112725785
Document Type :
Electronic Resource