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Increased muscle ubiquitin mRNA levels in gastric cancer patients

Authors :
Bossola, Maurizio
Muscaritoli, M
Costelli, P
Bellantone, Rocco Domenico Alfonso
Pacelli, Fabio
Busquets, S
Argilès, J
Lopez Soriano, Fj
Civello, Im
Baccino, Fm
Rossi Fanelli, F
Doglietto, Gb
Bossola, Maurizio (ORCID:0000-0003-1627-0235)
Bellantone, Rocco Domenico Alfonso (ORCID:0000-0002-0844-3469)
Pacelli, Fabio (ORCID:0000-0002-2013-6525)
Bossola, Maurizio
Muscaritoli, M
Costelli, P
Bellantone, Rocco Domenico Alfonso
Pacelli, Fabio
Busquets, S
Argilès, J
Lopez Soriano, Fj
Civello, Im
Baccino, Fm
Rossi Fanelli, F
Doglietto, Gb
Bossola, Maurizio (ORCID:0000-0003-1627-0235)
Bellantone, Rocco Domenico Alfonso (ORCID:0000-0002-0844-3469)
Pacelli, Fabio (ORCID:0000-0002-2013-6525)
Publication Year :
2001

Abstract

The intramuscular ATP-dependent ubiquitin (Ub)-proteasome proteolytic system is hyperactivated in experimental cancer cachexia. The present study aimed at verifying whether the expression of the muscle Ub mRNA is altered in patients with cancer. Total muscle RNA was extracted using the guanidinium isothiocyanate/phenol/chloroform method from rectus abdominis biopsies obtained intraoperatively from 20 gastric cancer (GC) patients and 10 subjects with benign abdominal diseases (CON) undergoing surgery. Ub mRNA levels were measured by northern blot analysis. Serum soluble tumor necrosis factor receptor (sTNFR) was measured by ELISA. Ub mRNA levels (arbitrary units, means +/- SD) were 2,345 +/- 195 in GC and 1,162 +/- 132 in CON (P = 0.0005). Ub mRNA levels directly correlated with disease stage (r = 0.608, P = 0.005), being 1,945 +/- 786 in stages I and II, 2,480 +/- 650 in stage III, and 3,799 +/- 66 in stage IV. Ub mRNA and sTNFR did not correlate with age and nutritional parameters. This study confirms experimental data indicating an overexpression of muscle Ub mRNA in cancer cachexia. Lack of correlation with nutritional status suggests that Ub activation in human cancer is an early feature that precedes any clinical sign of cachexia.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1105004252
Document Type :
Electronic Resource