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Structure-guided redesign of D-fructose-6-phosphate aldolase from E. coli: remarkable activity and selectivity towards acceptor substrates by two-point mutation

Authors :
Gutiérrez, Mariana
Parella, Teodor
Joglar Tamargo, Jesús
Bujons, Jordi
Clapés Saborit, Pere
Gutiérrez, Mariana
Parella, Teodor
Joglar Tamargo, Jesús
Bujons, Jordi
Clapés Saborit, Pere
Publication Year :
2011

Abstract

Structure-guided re-design of the acceptor binding site of D-fructose-6-phosphate aldolase from E. coli leads to the construction of FSA A129S/A165G double mutant with an activity between 5- to >900-fold higher than that of wild-type towards N-Cbz-aminoaldehyde derivatives.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.on1103379389
Document Type :
Electronic Resource