Cloning and Heterologous Expression of Phytase Gene from Pantoea sp. 3.5.1

© 2016, Springer Science+Business Media New York.Phytases (myo-inositol hexakisphosphate phosphohydrolase) catalyzes the stepwise hydrolysis of phosphate groups from phytic acid (myo-inositol hexakisphosphate) or its salt phytate. These enzymes could be potentially used for the stereospecific and efficient production of different myo-inositol phosphate isomers with therapeutic features. In the present study, we cloned the 1728 bp open reading frame encoding Pantoea sp. 3.5.1 phytase into the expression vector pET28a. The recombinant Escherichia coli BL21 pLysS pET28a/agpP strain expressing Pantoea sp. 3.5.1 AgpP phytase was obtained.