胃原発悪性リンパ腫症例のパラフィン包埋材料による免疫組織化学的再検討

We examined surgically resected specimens from 13 patients with gastrointestinal lymphoproliferative diseases using the immunogold-silver staining technique. They included 11 cases of gastric non-Hodgkin's lymphoma (NHL), one case of gastric reactive lymphoid hyperplasia and one of intestinal NHL, large cell type. The initial diagnosis of gastric NHLs were diffuse small cell type (5 cases), medium sized cell type (3 cases), mixed type (one case) and large cell type (2 cases). In paraffin sections, the presence of surface immunoglobulins in lymphocytes was shown. Using an image analysis system, OLYMPUS CIA-102, we estimated the monoclonality of individual cases. While all of medium sized or large cell type NHLs revealed monoclonality, three cases of 5 small cell type NHLs did not show the monoclonality. The result of this study suggested that the previous diagnosis of NHL, small cell type was still open to discussion. The histological diagnosis of gastrointestinal NHL should be amply confirmed by immunological or immunohistochemical studies.