Synthesis, characterization and biological activity of 3-aryl-6-(4-fluorobenzyl)-7H-thiazolo[3,2-b]-1,2,4-triazin-7-one derivatives as novel acetylcholinesterase inhibitors

Acetylcholinesterase (AChE) inhibitors play an important role in the treatment of Alzheimer's disease. To study the effect of the presence of a fluoro group on inhibitory activity against AChE, a series of 3-aryl-6-(4-fluorobenzyl)-7H-thiazolo[3,2]-1,2,4-triazin-7-one derivatives have been synthesized, in which a hydrogen atom has been substituted by a fluorine in the aromatic nucleus of 6-arylmethyl-3-phenyl-7H-thiazolo[3,2-b]-1,2,4-triazin-7-one derivatives, a series of compounds previously reported by us to have inhibitory activity against acetylcholinesterase. From the molecular docking studies, it was found that, in general, a key hydrogen bond of target compounds to the OH of Tyr341 and a close contact with relevant residues in the catalytic site are prerequisites for high inhibitory activity.