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Effect of viscogens on the kinetic response of a photoperturbed allosteric protein

Authors :
Waldauer, Steven A
Stucki-Buchli, Brigitte
Frey, Lukas
Hamm, Peter; https://orcid.org/0000-0003-1106-6032
Waldauer, Steven A
Stucki-Buchli, Brigitte
Frey, Lukas
Hamm, Peter; https://orcid.org/0000-0003-1106-6032
Source :
Waldauer, Steven A; Stucki-Buchli, Brigitte; Frey, Lukas; Hamm, Peter (2014). Effect of viscogens on the kinetic response of a photoperturbed allosteric protein. Journal of Chemical Physics, 141(22):514.
Publication Year :
2014

Abstract

By covalently binding a photoswitchable linker across the binding groove of the PDZ2 domain, a small conformational change can be photo-initiated that mimics the allosteric transition of the protein. The response of its binding groove is investigated with the help of ultrafast pump-probe IR spectroscopy from picoseconds to tens of microseconds. The temperature dependence of that response is compatible with diffusive dynamics on a rugged energy landscape without any prominent energy barrier. Furthermore, the dependence of the kinetics on the concentration of certain viscogens, sucrose, and glycerol, has been investigated. A pronounced viscosity dependence is observed that can be best fit by a power law, i.e., a fractional viscosity dependence. The change of kinetics when comparing sucrose with glycerol as viscogen, however, provides strong evidence that direct interactions of the viscogen molecule with the protein do play a role as well. This conclusion is supported by accompanying molecular dynamics simulations.

Details

Database :
OAIster
Journal :
Waldauer, Steven A; Stucki-Buchli, Brigitte; Frey, Lukas; Hamm, Peter (2014). Effect of viscogens on the kinetic response of a photoperturbed allosteric protein. Journal of Chemical Physics, 141(22):514.
Notes :
application/pdf, application/pdf, https://www.zora.uzh.ch/id/eprint/106218/7/1.4897975.pdf, English, English
Publication Type :
Electronic Resource
Accession number :
edsoai.ocn942503222
Document Type :
Electronic Resource