Controlled gene expression in primary Lgr5 organoid cultures

Authors :: Koo, B.K.
Stange, D.E.
Sato, T.
Karthaus, W.
Farin, H.F.
Huch, M.
van Es, J.H.
Clevers, H.
Koo, B.K.
Stange, D.E.
Sato, T.
Karthaus, W.
Farin, H.F.
Huch, M.
van Es, J.H.
Clevers, H.
Source :: Nature Methods vol.9 (2011) nr.1 p.81-83 [ISSN 1548-7091]
Publication Year :: 2011
Abstract: The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.<br />The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinase-inducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.

Database :: OAIster
Journal :: Nature Methods vol.9 (2011) nr.1 p.81-83 [ISSN 1548-7091]
Notes :: DOI: 10.1038/NMETH.1802, Nature Methods vol.9 (2011) nr.1 p.81-83 [ISSN 1548-7091], English
Publication Type :: Electronic Resource
Accession number :: edsoai.ocn931052529
Document Type :: Electronic Resource