Assessment of methods of detection of urban wastewaters estrogenic activity in order to develop a process of enzymatic degradation of wastewaters estrogenic endocrine disruptors

Methods of monitoring of estrogenicity in water were gathered, compared, and tested within thecontext of their practical use as measurement and design tools, in the development of a process ofdegradation of estrogenic endocrine disruptors. In this work, the focus was put on in vitro assays,with the use of analytical techniques as additional analysis when possible. Practically, from aliterature review, four methods that seemed most suitable to practical use required in a processdevelopment were tested: the Yeast Estrogen Screen assay, the Lyticase-assisted Yeast EstrogenScreen assay (LYES), the MMV-LUC assay and the HPLC-UV analytical method. Dose–responsecurves in response to estrogenic standard 17β-estradiol were compared. Bisphenol Aestrogenicity was measured by the methods as well. The model for the calculation of estradiolequivalents as measurements units was adapted. The methods were assessed in terms of rangesof detection, time of experiment, cost, ease of the experiment, reproducibility, etc. Based on thatassessment, the LYES assay was selected and successfully applied to the monitoring ofestrogenicity removal from 17β-estradiol and bisphenol A. More precisely, the bioassay allowedthe acquisition of kinetic curves for a laboratory-scaled process of estrogenicity removal byimmobilized enzymes in a continuous packed-bed reactor. The LYES assay was found to have areal methodological potential for scale-up and design of a treatment process. The HPLC-UVmethod showed good complementarity with the LYES assay for the monitoring of bisphenol Aconcentrations in parallel with estrogenicity, reporting no significant estrogenicity fromdegradation byproducts, among others.
SCOPUS: ar.j
info:eu-repo/semantics/published