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Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Authors :
Fytianos, Kleanthis
Rodriguez-Lorenzo, Laura
Clift, Martin J.D.
Blank, Fabian
Vanhecke, Dimitri
Garnier, Christophe von
Petri-Fink, Alke
Rothen-Rutishauser, Barbara
Fytianos, Kleanthis
Rodriguez-Lorenzo, Laura
Clift, Martin J.D.
Blank, Fabian
Vanhecke, Dimitri
Garnier, Christophe von
Petri-Fink, Alke
Rothen-Rutishauser, Barbara

Abstract

Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.ocn913448069
Document Type :
Electronic Resource