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Impaired hepatic antioxidant status in carbon tetrachloride intoxicated rats: An in vivo model for screening herbal extracts with antioxidant activities

Authors :
Ko, Kam Ming
Yick, Pak Kin
Chiu, Tillie
Hui, T.Y.
Cheng, Christopher Hon Ki
Kong, Yun Cheung
Ko, Kam Ming
Yick, Pak Kin
Chiu, Tillie
Hui, T.Y.
Cheng, Christopher Hon Ki
Kong, Yun Cheung
Publication Year :
1993

Abstract

In the present study, a drastic impairment in hepatic antioxidant status was observed in rats 24 h following oral administration of carbon tetrachloride as indicated by increases in the sensitivity of hepatic tissue homogenates to glutathione depletion and formation of thiobarbituric acid reactive substances following in vitro oxidative challenge, as well as decreases in tissue glutathione content and activities of glutathione reductase and Cu, Zn-superoxide dismutase. These hepatic alterations were accompanied by an increase in plasma alanine aminotransferase activities. Hepatoprotection afforded by pretreating carbon tetrachloride-intoxicated rats with a single dose of vitamin E (400 mg/kg, s.c.) or multiple doses of herbal extracts (100 mg/rat/day, p.o.) was evidenced by the significant reversal of hepatic antioxidant capacity and plasma alanine aminotransferase activity. While the depressed antioxidant enzyme activities were only marginally increased by vitamin E pretreatment, the herbal extract pretreatment significantly restored the activity of these enzymes. The measurement of hepatic antioxidant status in carbon tetrachloride intoxicated rats may provide a useful in vivo model for screening herbal extracts with antioxidant activities.

Details

Database :
OAIster
Notes :
English
Publication Type :
Electronic Resource
Accession number :
edsoai.ocn895575479
Document Type :
Electronic Resource