Quantitative determination of reducing sugars, oligosaccharides, and glycoproteins with [3H]borohydride

Authors :: Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104, USA; Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, Michigan 48104, USA.
McLean, Charles M.
Werner, D. A.
Aminoff, David
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48104, USA; Department of Internal Medicine (Simpson Memorial Institute), University of Michigan, Ann Arbor, Michigan 48104, USA.
McLean, Charles M.
Werner, D. A.
Aminoff, David
Publication Year :: 2006
Abstract: A very simple and direct radioactive isotope method has been described for the quantitative determination of reducing sugars. The method can be adapted to give any degree of sensitivity desired. The procedure is of general applicability to the determination of a large class of sugars. The stoichiometry of the reaction makes it a comprehensive procedure which greatly facilitates the analysis of sugars, oligosaccharides, and glycoproteins after acid and enzymic hydrolysis. Examples of the versatility of the method are given.

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