Multidisciplinary Analysis of Cyclophilin A Function in Human Breast Cancer

The goal of this project was to understand how cyclophilin A (CypA) regulates the prolactin receptor (PRLr) signaling. The data have demonstrated that CypA, serving as a molecular switch via its peptidyl-prolyl isomerase (PPI) activity, binds to and regulates the function of the PRLr. Furthermore, point mutagenesis analysis has implied that a single prolyl imide bond (Pro-334) within the X-Box motif of intracellular domain of the PRLr is most likely responsible for the observed effects of CypA on the PRLr signaling. Finally, we have conducted several proof-of-concept studies that may lead to novel anti-breast cancer agents.
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